Table of values for potential pigment combinations used in FRET

Potential combinations of pigments for proximity assays

 

Donor

Acceptor

Donor
Absorbance

(nm)

Acceptor
Emission

(nm)

Time
Resolved

Notes

Verified

R-PC

APC

540 - 620*

660 - 670

No

Naturally occurring donor-acceptor pair

No

Eu

APC

340

660 - 670

Yes

 

Yes

Eu

C-PC

340

635 - 650

Yes

Possibly brighter than APC

No

Eu

C-PC/APC tandem

340

660 - 670

Yes

Possibly brighter than APC

No

Eu

APC/Cy7 tandem

340

750 - 790

Yes

 

No

APC

Cy7

615 - 655*

750 - 790

No

Successful tandem

No

R-PE

APC

475 - 575*

660 - 670

No

Successful tandem

No

R-PE

R-PC

475 - 575*

630 - 650

No

Naturally occurring donor-acceptor pair

No

R-PE

Cy5

475 - 575*

660 - 680

No

Successful tandem

Yes

R-PC

Cy5

540 - 620*

660 - 680

No

 

No

Tb

R-PE

340

570 - 590

Yes

 

Yes

Tb

B-PE

340

570 - 590

Yes

Possibly brighter than R-PE

No

Tb

R-PC

340

630 - 650

Yes

 

No

Tb

R-PC/APC tandem

340

660 - 670

Yes

 

No

Tb

R-PE/APC tandem

340

660 - 670

Yes

 

No

Tb

R-PE/TxRed tandem

340

600 - 640

Yes

Successful tandem

No


 

Key: 

R-PC - R-Phycocyanin

Eu - Europium

APC - Allophycocyanin

R-PE - R-Phycoerythrin

Tb - Terbium

C-PC - C-Phycocyanin

B-PE - B-Phycoerythrin

 

APC, R-PE, C-PC & B-PE are available from ProZyme

Texas Red®  is a trademark of Molecular Probes, Eugene, Oregon, USA

Cy5 & Cy7 are tradenames of Amersham - Pharmacia Biotech, Uppsala, Sweden

* May also be excited in UV