Streptavidin Agarose Purification Conditions

I am using a Streptavidin agarose system to affinity purify biotin labeled, outer membrane proteins of a gram negative bacterium. Could you provide info regarding preparation of buffers and the reaction and elution conditions of this system?


The high affinity of streptavidin for biotin precludes using streptavidin-agarose for binding and eluting biotinylated proteins, because there is no way to elute the proteins without denaturing them and the streptavidin. Streptavidin-agarose has been used for affinity purification, by binding a biotinylated affinity ligand. Examples include binding biotinylated antibodies to streptavidin-agarose for immunoaffinity purification of antigens.

Refer to our Streptavidin-agarose Technical Data Sheet (Product Code CJ30R). Several examples of such procedures are cited.