Desalting columns (Columns A & B) may be purchased from GE Healthcare (PD-10, catalog #17-0851-01). Initially we used columns from Pierce Chemical (catalog #43232), so you may see some difference in the appearance from each supplier. Column material is cross-linked dextran. Please note that the columns must be equilibrated before use.
Columns A & B may be re-used several times. To prepare a column for initial use or re-use, simply wash it with 5 - 10 column volumes of the appropriate buffer (recipes for buffers used in the Conjugation Protocol may be found in the Appendix of the PJ31K manual). We have used these columns 2 - 4 times over the course of a day, but we havent kept used columns for longer periods. You might be able to do so, but microbial growth is a concern.
Spin Column D may be purchased separately from Bio-Rad (Bio-Spin P-6 Gel Columns, catalog #7326002).
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