PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. 

Prior denaturation of the glycoprotein substrate by treatment with heat/SDS greatly enhances the rate and reliability of N-glycan removal, although at higher concentrations the enzyme can remove intact glycans from glycoproteins in native conditions. Rapid (5-min) deglycosylation with PNGase F is part of our Gly-X sample prep platform.

We carry a number of different formulations and pack sizes of PNGase F:

Product

Concentration

Recombinant

EDTA

GKE-5006A, GKE-5006B, GKE-5006D

≥ 2.5 U/ml

1mM

GKE-5016A, GKE-5016B, GKE-5016D

≥ 2.5 U/ml

0

GKE-5010B, GKE-5016D

≥ 10 U/ml

1 mM

GKE-5020B, GKE-5020D

≥ 10 U/ml

0

GKE-5003

≥ 2.0 U/ml

 

5 mM

One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 and 37°C.

Buffers and denaturant are not supplied with 1 U "D" pack sizes, but can be made available on request.

EDTA was included historically in native PNGase F formulations as a stabilizer. For the GKE-5020 formulation we revisited the idea, due to requests from customers for a PNGase F formulation without EDTA. We believe that stability is not as much of a concern with the recombinant form of the enzyme.

N-Glycanase® (PNGase F), 100 mU [GKE-5006A]

$ 244.00

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F), 200 mU [GKE-5006B]

$ 446.00

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F), 1 U [GKE-5006D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase-PLUS® (PNGase F), 400 mU [GKE-5010B]

$ 1,060.00

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase-PLUS® (PNGase F), 1 U [GKE-5010D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F, EDTA-Free), 100 mU [GKE-5016A]

$ 244.00

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F, EDTA-Free), 200 mU [GKE-5016B]

$ 446.00

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F, EDTA-Free), 1 U [GKE-5016D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® ULTRA (PNGase F), 400 mU [GKE-5020B]

$ 1,060.00

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® ULTRA (PNGase F), 1 U [GKE-5020D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

Glyko® PNGase F (native), 100 mU [GKE-5003]

$ 200.00

Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc...