Cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans. Endoglycosidase F2, endo-beta-N-acetylglucosaminidase.
Recombinant gene from Elizabethkingia meningosepticum, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum1.
The enzyme cleaves within the di-N-acetyl chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides.
Figure 1: Cleavage specificity for GKE-5008 Endo F2.
R1: N & C substitution other than H
R2: may be H or the rest of an oligosaccharide
R3: may be H or α(1-6)-linked fucose
Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins.
WS0161 5X Reaction Buffer, which when diluted gives 200 mM sodium acetate pH 4.5.
10 mM sodium acetate pH 4.5, 25 mM NaCl, 0.02% sodium azide
One milliunit is defined as the amount of enzyme that will hydrolyze 1 nmole of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C.
Size: 6 mU (60 µl)
Concentration: ≥ 100 mU/ml
Product Code: GKX-5008
1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST. Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1287-93.
EC Number: 18.104.22.168
CAS Number: 37278-88-9
Product Safety Documentation for GKE-5008: