Glyko® Endo H [GKE-5002]

Price:
$ 300.00

Description

Cleaves within the N,N'-diacetylchitobiose (GlcNAc-GlcNAc) core of high mannose and some hybrid type N-glycans. Endoglycosidase H, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase. 

Source:
Recombinant gene from Streptomyces plicatus1, expressed in E. coli

Specificity:
Cleaves between the GlcNAc residues of the chitobiose (GlcNAc-GlcNAc) core of N-linked glycans. Oligomannose & some hybrid type N-glycans are released from glycoproteins, leaving a single GlcNAc attached to asparagine. This specificity is distinct from Endo F2 (GKE-5008) which also cleaves biantennary complex type N-glycans, and PNGase F (GKE-5006) which releases most N-glycans by cleaving between the innermost GlcNAc and asparagine.

 

Figure 1: Cleavage specificity for GKE-5002 Endo H.

R1: Man2-150
R2: H, monosaccharide or oligosaccharide at C2 and/or C4
R3: GlcNAc ± Fuc, Asn or Dol-pp

Applications:
Selective release of high mannose N-linked oligosaccharides from glycopeptides/ glycoproteins. The enzyme has found extensive use in the characterization of glycoproteins, dolichol-linked glycans and the biosynthetic pathway for N-glycosylation.

Ships with: 

  • WS0079 5x Reaction Buffer for GKE-5002 (500 mM Sodium Citrate/Phosphate, pH 5.5) 
  • WS0012 Denaturation Solution (2% SDS/1M βME)
  • WS0013 Detergent Solution (15% Detergent Solution)

Formulation:
50 mM sodium phosphate pH 7.5, 25 mM EDTA,  and 0.05% sodium azide

pH optimum:
5.5

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 60 μmoles of denatured ribonuclease B in 1 hour at 37°C, pH 5.5.

Size: 30 mU (200 µl)

Concentration: ≥ 0.15 U/ml

Product Code: GKE-5002

1. Robbins PW, Trimble RB, Wirth DF, Hering C, Maley F, Maley GF, Das R, Gibson BW, Royal N, Biemann K. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosaminidase H.  1984 Jun 25;259(12):7577-83.

Tech Info

EC Number: 3.2.1.96

CAS Number: 37278-88-9

Database entries:

BRENDA | ExPASy | ExplorEnz | IntEnzIUBMB | KEGG | MetaCyc | PDB

Safety

Product Safety Documentation for GKE-5002:

Product/Part No. Description
GKE-5002 Glyko® Endo H 
WS0079

US-5x Reaction Buffer

GB-5x Reaction Buffer

WS0012  Denaturation Solution 
WS0013 Detergent Solution 

 

 

 

 

 

 

 

 


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